薬剤耐性結核の脅威

タイトル: Drug resistant Mycobacterium tuberculosis strains have altered cell envelope hydrophobicity that influences infection outcomes in human macrophages

概要: Mycobacterium tuberculosis (M.tb), the causative agent of tuberculosis (TB), is considered one of the top infectious killers in the world. In recent decades, drug resistant (DR) strains of M.tb have emerged that make TB even more difficult to treat and pose a threat to public health. M.tb has a complex cell envelope that provides protection to the bacterium from chemotherapeutic agents. Although M.tb cell envelope lipids have been studied for decades, very little is known about how their levels change in relation to drug resistance. In this study, we examined changes in the cell envelope lipids [namely, phthiocerol dimycocerosates (PDIMs)], glycolipids [phosphatidyl-myo-inositol mannosides (PIMs)], and the PIM associated lipoglycans [lipomannan (LM); mannose-capped lipoarabinomannan (ManLAM)] of 11 M.tb strains that range from drug susceptible (DS) to multi-drug resistant (MDR) to pre-extensively drug resistant (pre-XDR). We show that there was an increase in the PDIMs:PIMs ratio as drug resistance increases, and provide evidence of PDIM species only present in the DR-M.tb strains studied. Overall, the LM and ManLAM cell envelope levels did not differ between DS- and DR-M.tb strains, but ManLAM surface exposure proportionally increased with drug resistance. Evaluation of host-pathogen interactions revealed that DR-M.tb strains have decreased association with human macrophages compared to DS strains. The pre-XDR M.tb strain with the largest PDIMs:PIMs ratio had decreased uptake, but increased intracellular growth rate at early time points post-infection when compared to the DS-M.tb strain H37Rv. These findings suggest that PDIMs may play an important role in drug resistance and that this observed increase in hydrophobic cell envelope lipids on the DR-M.tb strains studied may influence M.tb-host interactions. AUTHOR SUMMARYTuberculosis (TB) is a leading cause of death due to an infectious organism and is caused by the bacteria Mycobacterium tuberculosis (M.tb). Drug resistant (DR) forms of TB have emerged over the past few decades which make the disease even more difficult to diagnose and treat. Currently, there is very little known about how the bacteria changes as it becomes more drug resistant. Here, we used biochemical techniques to study differences in the M.tb cell envelope across drug resistance categories. We examined 11 M.tb strains that range from drug-susceptible (DS) to multi-drug resistant (MDR) to pre-extensively drug resistant (pre-XDR) and observed that levels of hydrophobic phthiocerol dimycocerosates were increased and levels of hydrophilic higher-order phosphatidyl-myo-inositol mannosides were decreased in DR-M.tb strains compared to DS strains. We also found that DR-M.tb strains had decreased association with human macrophages, and that the pre-XDR-M.tb strain with the highest ratio of hydrophobic to hydrophilic lipids had decreased uptake but increased intracellular growth in macrophages at early timepoints after infection. Our study provides exciting insights into changes in the cell envelope composition of DR-M.tb strains and how these changes may influence infection outcomes in human macrophages.