inkube: A New Way to Study Neurons
Discover how inkube is changing brain research and neuron observation.
Benedikt Maurer, Selina Fassbind, Tobias Ruff, Jens Duru, Giusy Spacone, Theo Rodde, János Vörös, Stephan J. Ihle
― 5 min read
Table of Contents
inkube is a new, affordable device designed to study how brain cells, or Neurons, behave and interact with each other. Think of it as a high-tech fish tank for neurons, where researchers can watch them swim around, make friends, and even react to different stimuli—all while keeping them cozy and comfortable. inkube allows scientists to manipulate the environment and observe how these microscopic creatures react, paving the way for breakthroughs in understanding brain function and treating neurological diseases.
Why study neurons?
Neurons are the building blocks of the brain, responsible for sending signals throughout the body. When these neurons misbehave, it can lead to various neurological diseases, like Alzheimer's or Parkinson's. By studying how neurons work, scientists hope to unlock the secrets of the brain and develop new treatments. The challenge has always been figuring out a reliable way to observe these tiny cells while maintaining their natural environment.
The challenges of studying neurons
Studying neurons isn't as simple as watching fish swim. The brain is a complex organ, and various factors—like temperature, humidity, and nutrients—can affect how neurons behave. It can be a bit like trying to bake a cake while the oven temperature keeps changing. To get consistent results, researchers need to create a stable environment for their neurons. This is where inkube comes in.
What can inkube do?
inkube provides researchers with the ability to control many factors that influence neuron behavior:
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Temperature Control: Just like we feel more comfortable at certain temperatures, neurons have their preferences too. inkube ensures that the temperature remains just right for the neurons.
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Humidity Control: Too dry? Too humid? Not in inkube! It creates the perfect atmosphere for neurons to thrive.
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CO2 Control: Neurons also need to breathe! inkube keeps the right levels of CO2 to ensure the cells stay happy and healthy.
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Liquid Management: inkube can add or remove liquids from the neuron environment automatically, ensuring that they have the right nutrients without the researchers needing to get involved every five minutes.
The setup of inkube
inkube consists of several layers, each responsible for a specific function, kind of like a well-organized sandwich:
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Incubation Layer: This is where the neurons live. It contains the neurons and all the equipment needed to measure their activity. It’s like their own little penthouse suite.
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Ventilation Layer: This layer helps keep everything cool. It allows air to circulate, which helps with temperature regulation—sort of like a mini air conditioner.
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Electronics Layer: Here lies all the high-tech gadgets and gizmos that make inkube work. This is where the magic happens!
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Fluidics Layer: The bottom layer is responsible for managing liquids. It ensures that everything flows smoothly, kind of like a well-oiled machine.
Measuring neuron activity
So, how do we know if our neurons are partying or just lounging around?
inkube uses a special kind of technology called Microelectrode Arrays (MEAs) to listen in on neuron conversations. These are tiny sensors placed among the neurons that can pick up the electrical signals they send. By recording these signals, scientists can see how active the neurons are and how they respond to different conditions.
The brilliance of open-source
inkube is part of the open hardware movement, which means that all the plans, instructions, and parts are available to anyone who wants to build their own. This approach promotes creativity and collaboration in science. Researchers from various places can adapt inkube to fit their specific needs, making it a versatile tool for studying brain activity.
Experiments and results
Researchers have already begun using inkube for various experiments. For instance, they’ve tested how changing the temperature affects neuron activity. Spoiler alert: neurons tend to get more active as it gets warmer! They’ve also looked at what happens when the liquid level in the MEAs decreases, simulating evaporation. Results showed that neurons can slow down their activity when the environment becomes less stable.
A glimpse into the future
inkube represents a step forward in the quest to understand the brain. With its ability to control multiple factors at once, it opens up new avenues for research. Imagine a future where we can better understand how memories form or how diseases develop. It’s not just about understanding the brain; it’s about creating a healthier future.
Why you should care
While the fine details of neurons and brain activity might seem distant from everyday life, the implications of understanding how our brains work are profound. Whether it's developing new treatments for neurodegenerative diseases, improving mental health therapies, or simply making sense of why we think the way we do, tools like inkube are crucial in unlocking the mysteries of the human brain.
Conclusion
inkube is leading the way in brain research, offering a new platform for scientists to study neurons in a controlled, reproducible environment. With its affordable and flexible design, inkube is set to become a valuable asset for researchers striving to understand one of the most complex organs in our body. It’s a device that not only aims to shake things up in the lab but also carries the hope of making significant contributions to the world of neuroscience. Who knew that something designed for tiny brain cells could have such a big impact on human health? Now that’s something to ponder over a cup of coffee!
Original Source
Title: Inkube: An all-in-one solution for neuron culturing, electrophysiology, and fluidic exchange
Abstract: Culturing neuronal networks in vitro is a tedious and time-consuming endeavor. In addition, how the composition of the culture medium and environmental variables such as temperature, osmolarity, and pH affect the spiking behavior of neuronal cultures is difficult to study using electrophysiology. In this work, we present "inkube", an incubation system that has been combined with an electrophysiology setup and a fully automatic perfusion system. This setup allows for the precise measurement and control of the temperature of up to 4 microelectrode arrays (MEAs) in parallel. In addition, neuronal activity can be electrically induced and recorded from the MEAs. inkube can continuously monitor the medium level to automatically readjust osmolarity. Using inkubes unique capability to precisely control the environmental variables of a neural culture, we found that medium evaporation influences the spiking response. Moreover, decreasing medium temperature by only 1.5{degrees}C significantly affected spike latency, a measure commonly used to show plasticity in in vitro experiments. We finally provide a proof-of-concept experiment for drug screening applications, where inkube automatically and precisely varies the concentration of magnesium ions in the medium. Given its high level of autonomy, the system can record, stimulate, and control the medium continuously without user intervention. Both the hardware and the software of inkube are completely open-source. HighlightsO_LILow-cost, open-hardware/open-software electrophysiology setup C_LIO_LIFull incubation solution: Temperature, CO2, and humidity control C_LIO_LIPerfusion system for automatic fluidic exchange and drug testing with volume feedback C_LI
Authors: Benedikt Maurer, Selina Fassbind, Tobias Ruff, Jens Duru, Giusy Spacone, Theo Rodde, János Vörös, Stephan J. Ihle
Last Update: 2024-12-11 00:00:00
Language: English
Source URL: https://www.biorxiv.org/content/10.1101/2024.12.06.627248
Source PDF: https://www.biorxiv.org/content/10.1101/2024.12.06.627248.full.pdf
Licence: https://creativecommons.org/licenses/by-nc/4.0/
Changes: This summary was created with assistance from AI and may have inaccuracies. For accurate information, please refer to the original source documents linked here.
Thank you to biorxiv for use of its open access interoperability.