How Dairy Products Help Track H5N1 Virus
Retail dairy sampling reveals crucial insights on zoonotic disease monitoring.
Andrew J. Lail, William C. Vuyk, Heather Machkovech, Nicholas R. Minor, Nura R. Hassan, Rhea Dalvie, Isla E. Emmen, Sydney Wolf, Annabelle Kalweit, Nancy Wilson, Christina M. Newman, Patrick Barros Tiburcio, Andrea Weiler, Thomas C. Friedrich, David H. O’Connor
― 6 min read
Table of Contents
- The Importance of One Health
- H5N1 and Its Impact
- How Milk Became a Focus for Surveillance
- Testing for HPAI in Dairy Products
- The Tiled-Amplicon Approach
- Findings from the Study
- The Broader Implications of the Research
- The Benefits of Accessible Sampling
- Limitations of Retail Milk Sampling
- The Need for More Comprehensive Research
- Conclusion: Lessons Learned and the Future
- Original Source
- Reference Links
In our interconnected world, the health of people, animals, and the environment is all linked together. This is the idea behind the One Health approach. It says that everything is connected, from the tiny germs in our bodies to the big ecosystems outside. When one part is affected, it can have a ripple effect on the others.
The Importance of One Health
Recently, we've seen how important this approach is, especially with diseases that jump from animals to humans, known as zoonotic diseases. These diseases can cause serious outbreaks and pandemics. This is why experts are focusing on ways to study and prevent such diseases.
One key feature of the One Health strategy is the need for clear communication and collaboration between different areas of health. This includes sharing information and working together to improve our response to emerging infectious diseases. It's like a team sport where everyone needs to play their part to win.
H5N1 and Its Impact
One of the diseases that has raised alarms is H5N1, a strain of bird flu that affects both birds and mammals. In 2021, a dangerous version of H5N1 was spotted in North America, impacting wildlife and poultry. Unfortunately, this resulted in many deaths among birds and livestock. By March 2024, a new outbreak of H5N1 was confirmed in Dairy cattle across several states. This raised concerns due to the potential for transmission to humans, particularly dairy workers.
Surveillance
How Milk Became a Focus forWhy milk, you might ask? Well, it's a food product that many people consume. Scientists decided to check if H5N1 was present in milk from retail sources. Collecting samples of milk from grocery stores could provide a clearer picture of where the virus was spreading, especially in areas where checking individual farms was tough.
In commercial dairy operations, milk from multiple cows is gathered, making it easier for scientists to test large samples without having to go farm by farm. Though pasteurization (a process that kills germs in milk) usually destroys the virus, scientists found that the genetic material of the H5N1 virus could still be detected.
Testing for HPAI in Dairy Products
Over a two-month period, researchers collected 66 samples of different dairy products from various states. They found that out of all these samples, some tested positive for H5N1 genetic material. To figure out how many samples were contaminated, they used a method called RT-qPCR. This technique helped them detect the virus's genetic fingerprints.
It turns out that retail dairy products, like milk and yogurt, can be useful for tracking the H5N1 virus. Even though pasteurization makes the virus harmless, researchers can still learn a lot by examining the milk for genetic markers of H5N1.
The Tiled-Amplicon Approach
To look deeper into the virus, researchers used a method called tiled-amplicon sequencing. This fancy term means they broke the virus's genetic code into chunks to analyze it better. By doing this, they were able to get a good portion of the virus’s code from the positive samples.
They found that some of the genetic sequences matched those of the H5N1 virus causing the outbreaks, allowing them to understand how the virus was evolving and spreading.
Findings from the Study
From the samples taken, researchers were able to confirm which states had H5N1 Contamination in their dairy products. The results were shared publicly, helping state health departments and other researchers respond swiftly to the outbreak.
Interestingly, the study revealed that while most samples had some sort of viral genetic material, not all were equally contaminated. Some samples showed higher strengths of the virus’s genetic material than others. Researchers analyzed these differences to learn more about the spread and severity of the outbreaks.
The Broader Implications of the Research
The findings from retail dairy sampling proved significant for several reasons. First, they revealed gaps in testing and surveillance systems. Many states were not regularly checking for H5N1 in dairy products, which could lead to delays in identifying outbreaks.
Second, the study highlighted the potential for using regular food products as a surveillance tool. By checking items that people commonly buy, researchers can keep tabs on viruses without relying solely on farm inspections.
The Benefits of Accessible Sampling
Collecting samples from grocery stores is not just more convenient; it’s also cost-effective. Given that dairy products are widely distributed, testing them increases the chances of catching potential outbreaks before they spread further.
Plus, because sampling can be done in basic laboratories, smaller testing facilities that don’t have high-security features can also participate in monitoring for H5N1. This democratizes the process, making it more inclusive for researchers across the board.
Limitations of Retail Milk Sampling
However, not every dairy product is created equal. A large percentage of milk produced goes into making cheese and other products rather than being sold as fluid milk. This means sampling retail milk might miss potential outbreaks happening on farms focused on manufacturing.
Moreover, when multiple sources are combined in a product, it can be tough to trace back to the original farm. The complexity increases if various ingredients come from different places, making it hard to pinpoint where the virus might have come from.
The Need for More Comprehensive Research
Researchers have acknowledged that more needs to be done to enhance the understanding of how HPAI spreads. States have different rules about testing, which can leave gaps in monitoring. The aim is to improve these practices based on findings from studies like this one.
Going forward, researchers can implement broader policies for testing and monitoring that can help ensure food safety and public health.
Conclusion: Lessons Learned and the Future
What’s the takeaway from all this work? For one, the One Health approach is not just a theory; it’s a practical framework that can be applied to real-world problems. Examining the connection between animal health, public health, and food safety can lead to better outcomes for everyone.
As we deal with outbreaks like H5N1, it’s crucial to think creatively about how we can monitor and respond. Retail dairy products offer a unique avenue for scientists to detect and analyze diseases, broadening the toolbox available for public health protection.
And who knows? Maybe the next big breakthrough in monitoring zoonotic diseases will come from the dairy aisle of your local store. Just think of it: while you’re grabbing your morning milk, scientists might be doing the detective work to keep everyone safe. So, the next time you pour milk into your cereal, remember that it might hold more than just breakfast; it could be a key piece in keeping diseases at bay.
In this ever-connected world, every small step counts, and every glass of milk could be part of a larger solution. So, raise your cup to science, collaboration, and the innovative ways we can protect our health and that of our furry friends!
Original Source
Title: Pasteurized retail dairy enables genomic surveillance of H5N1 avian influenza virus in United States cattle
Abstract: Highly pathogenic avian influenza (HPAI) viruses with H5 hemagglutinin (HA) genes (clade 2.3.4.4b) are causing an ongoing panzootic in wild birds. Circulation of these viruses is associated with spillover infections in multiple species of mammals, including a large, unprecedented outbreak in American dairy cattle. With limited testing of dairy herds, there is an unmet need for genomic surveillance. Infected cattle can shed high amounts of HPAI H5N1 viruses in milk, allowing detection in pasteurized retail dairy samples. Over a 2-month sampling period in one Midwestern city, we obtained dairy products processed in 20 different states. Here we demonstrate that a tiled-amplicon sequencing approach produced over 90% genome coverage at greater than 20x depth from 5 of 13 viral RNA positive samples. A combination of RT-qPCR testing and sequencing from retail dairy products can be a useful component of a One Health framework for responding to the avian influenza outbreak in cattle.
Authors: Andrew J. Lail, William C. Vuyk, Heather Machkovech, Nicholas R. Minor, Nura R. Hassan, Rhea Dalvie, Isla E. Emmen, Sydney Wolf, Annabelle Kalweit, Nancy Wilson, Christina M. Newman, Patrick Barros Tiburcio, Andrea Weiler, Thomas C. Friedrich, David H. O’Connor
Last Update: 2024-12-13 00:00:00
Language: English
Source URL: https://www.medrxiv.org/content/10.1101/2024.12.12.24318872
Source PDF: https://www.medrxiv.org/content/10.1101/2024.12.12.24318872.full.pdf
Licence: https://creativecommons.org/licenses/by/4.0/
Changes: This summary was created with assistance from AI and may have inaccuracies. For accurate information, please refer to the original source documents linked here.
Thank you to medrxiv for use of its open access interoperability.
Reference Links
- https://www.idtdna.com/pages/tools/primerquest
- https://github.com/andersen-lab/avian-influenza
- https://dx.doi.org/10.17504/protocols.io.kqdg322kpv25/v1
- https://www.ncbi.nlm.nih.gov/bioproject/
- https://github.com/dholab/Lail-et-al-2024-analysis-pipeline
- https://github.com/dholab/Lail-et-al-2024-analysis-pipeline/blob/main/pyproject.toml
- https://clades.nextstrain.org
- https://nextstrain.org/avian-flu/h5n1-cattle-outbreak/genome