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New Instrument Aims to Detect Life on Ocean Moons

Researchers advance technology to search for life on moons like Enceladus and Europa.

― 7 min read


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Recent scientific advancements have made it possible to study Ocean Worlds, such as Enceladus and Europa, which are moons in our solar system that may have conditions suitable for life. This has led researchers to look for signs of life on these moons. One method of searching for life involves looking for specific molecules that are the building blocks of life as we know it, particularly Amino Acids.

Amino acids are important because they are the main components of proteins, which are essential for living organisms. Some studies have shown that many amino acids can be found in meteorites, which suggests that these molecules can form without help from life and might not only come from Earth.

However, when scientists analyze amino acids found in meteorites, they notice that some patterns are different from what we see in living systems. For example, meteorites often have a lot of the simplest amino acid called glycine and have a mix of amino acids called racemic mixtures. In living organisms, more complex amino acids are usually found, and there is often a preference for a specific form of amino acids called homochirality.

These differences in the types and arrangements of amino acids could help scientists figure out whether certain molecules come from living beings or not. To find these signs of life in Ocean Worlds, scientists need very sensitive instruments that can detect small amounts of amino acids and other organic molecules.

Challenges in Life Detection Instruments

The instruments used in this search need to be flexible to work with different types of biochemistry. They also must be small, light, and durable since they will be sent into space, where conditions can be extreme-like high radiation, varying temperatures, and low Gravity.

One promising type of technology involves devices called solid-state nanogaps. These are tiny structures that can be made in materials like silicon or graphene. Nanogaps can detect single molecules, including nucleic acids and proteins, because they are very precise and sensitive.

One of these devices, called the Electronic Life Detection Instrument for Enceladus/Europa (ELIE), uses nanogap sensors based on a technology called quantum electronic tunneling. This allows it to detect individual molecules and was shown to successfully identify amino acids and other important compounds.

The initial version of ELIE, which was less advanced, could detect the amino acid L-proline. However, it had limitations since it needed manual adjustments to maintain the nanogap, which could only work for short periods.

Advancements in ELIE Technology

Recognizing the limitations of the first version, a new prototype of ELIE was developed. This next-generation model includes an automated mechanism that can control the gap size in real-time, making it much easier to use during experiments. This prototype was tested in a special flight that simulated different levels of gravity to see how well it could detect L-proline in changing conditions.

During the flight tests, the new ELIE prototype was put through various phases of gravity, including zero gravity and conditions similar to those on Mars and the Moon. Researchers compared its performance in flight with similar tests done on the ground.

The results showed that ELIE can keep working under different gravitational conditions and still function well after multiple changes in gravity. This successful testing is crucial for future missions that aim to detect potential signs of life on other celestial bodies.

Understanding the Instrument's Structure

The ELIE prototype consists of several components working together, including a sensitive amplifier and a chip that contains the nanogap. The chip is designed to create a very tiny gap between two electrodes, which allows it to detect the flow of electrons when molecules pass through. This is important for measuring how well the instrument works.

Before each experiment, the chip is prepared by rinsing it and adjusting the gap size accurately using the piezo actuator. Once the gap is formed, the researchers introduce a solution containing L-proline and measure electrical signals for a set period. During this time, they monitor how the instrument responds to the passing molecules.

Data from the tests-both on the ground and in flight-show how many molecules were detected and how the electrical signals changed. This information is crucial to understand the performance of the device.

Analyzing Data from the Experiments

The data collected during the flight tests was filtered and analyzed to identify relevant signals and distinguish them from background Noise. Researchers used specific methods to recognize actual events related to molecules passing through the gap, helping to differentiate these from random noise in the data.

One interesting finding was that the device detected many L-proline events, particularly during the zero-gravity parabolas. However, the number of detected events varied depending on the phase of the flight, indicating that different gravitational conditions can affect how well the instrument performs.

The results from both flight and ground tests were compared to previous experiments. Scientists analyzed how these conditions influenced the instrument's efficiency in detecting L-proline and other important compounds.

Challenges Faced During Testing

Despite the successful tests and promising results, the new ELIE prototype faced some challenges. One major issue was the level of noise affecting the data. Since the electrical signals from the molecules are very small, background noise can sometimes obscure the signals, leading to potential misunderstandings about the presence of molecules.

Noise could come from various sources, including the power supply used during the flight. Different power sources can introduce varied levels of noise, impacting the quality of the signals detected by the instrument. Additionally, the surrounding environment-such as temperature, pressure, and vibrations-could further contribute to noise levels.

Another challenge involved the stability of the nanogap during tests. Because the chip could not be replaced during the flight, any degradation in the gap between tests could lead to less accurate readings.

To address these limitations, future improvements are planned that will focus on reducing noise, enhancing the stability and structure of the nanogap, and refining the design of the ELIE prototype to make it lighter and more efficient for space missions.

Future Directions for ELIE

With ongoing efforts, researchers aim to enhance the ELIE instrument for future life-detection missions to Ocean Worlds. The ability to detect individual molecules like L-proline under various gravitational conditions is a significant advancement towards using solid-state technology for biosignature detection.

The automatic control of the nanogap size is a crucial improvement that addresses previous challenges. However, work remains to reduce noise levels and further refine the design to ensure that the device is compact and lightweight enough for space missions.

In addition, shifting from manual sample loading to an automatic system will allow the instrument to analyze larger sample volumes without needing direct human interaction. This upgrade will significantly enhance its capability to work in space.

As the ELIE prototype continues to develop and improve, it has the potential to become a sophisticated tool for detecting life on other planets and moons. Future missions, like the Orbilander mission to Enceladus, planned for 2038, could utilize this technology to conduct real-time analyses for potential biosignatures.

By building upon the successes and lessons learned from previous tests, the ELIE prototype stands ready to contribute to our understanding of life outside Earth. The mission to uncover the mysteries of Ocean Worlds continues, and ELIE may play a crucial role in this exciting exploration.

Original Source

Title: Nanogap Solid-State Single-Molecule Detection at Mars, Europa, and Microgravity Conditions

Abstract: Solid-state nanogap systems are an emerging technology for in-situ life detection due to their single-molecule resolution of a wide range of biomolecules, including amino acids and informational polymers, at the parts per billion to trillion level. By targeting the abundance distributions of organic molecules, this technology is a candidate for detecting ancient and extant life and discriminating between biotic and abiotic organics on future planetary missions to Mars and icy moons such as Enceladus and Europa. A benchtop system developed at Osaka University has a proven ability to detect and discriminate among single amino acids, RNA, and DNA using nanogap chips. The Electronic Life-detection Instrument for Enceladus/Europa (ELIE) prototype was subsequently developed to make this technology viable for space instrumentation through the simplification of electronics, reduction of size and weight, and automation of gap formation. Initial ground testing using a manually formed nanogap with the first ELIE prototype detected the amino acid L-proline. However, this manual adjustment approach posed limitations in maintaining a consistent gap size. To address this challenge, we integrated an automated piezo actuator to enable real-time gap control, permitting single-molecule identification of a target amino acid, L-proline, under reduced gravity (g), including Mars (g = 0.378), Europa or Lunar (g = 0.166), and microgravity conditions (g = 0.03-0.06), as validated through parabolic flight testing. Power supply noise and experimental constraints of the experiment design limited data collection to short segments of good-quality data. Nevertheless, the subsequent analysis of detected events within these segments revealed a consistent system performance and a controlled gap size across the different accelerations. This finding highlights the systems resilience to physical vibrations. Future goals are to progress the instrument towards technology readiness level 4 with further reductions of size and mass, lower noise, and additional system automation. With further development, ELIE has the potential to be an autonomous and sensitive single-molecule detection instrument for deployment throughout the solar system.

Authors: José L Ramírez-Colón, J. L. Ramirez-Colon, E. Johnson, D. Duzdevich, S. Lee, J. Soderblom, M. T. Zuber, M. Taniguchi, T. Ohshiro, Y. Komoto, C. E. Carr

Last Update: 2024-03-02 00:00:00

Language: English

Source URL: https://www.biorxiv.org/content/10.1101/2024.02.29.582359

Source PDF: https://www.biorxiv.org/content/10.1101/2024.02.29.582359.full.pdf

Licence: https://creativecommons.org/licenses/by-nc/4.0/

Changes: This summary was created with assistance from AI and may have inaccuracies. For accurate information, please refer to the original source documents linked here.

Thank you to biorxiv for use of its open access interoperability.

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