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New Breeding Method Boosts Marmoset Success

Female choice in marmoset breeding leads to higher pregnancy rates.

― 5 min read


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Table of Contents

Common marmosets are small monkeys that have become popular in scientific research. They are valued for their interesting ways of reproducing and developing. Researchers are especially interested in baby marmosets because they help us see how young animals grow and behave socially, much like humans do with families. Marmosets live in groups, and they take care of each other’s young. Their brains share some similarities with human brains, making them useful for studying emotional and thinking problems in people.

However, the popularity of marmosets for research has led to a shortage of these monkeys available for laboratories worldwide. Buying marmosets can be very expensive, with prices around $12,000 per monkey. Because of this, many research facilities have started Breeding their own marmosets. Breeding marmosets can be tough, even though they generally live a long time. Female marmosets often do not reproduce well because they may not ovulate properly. This can happen when they are under stress or if they are not the dominant female in the group. Sometimes, Females may try to breed but have very few babies survive.

Pairing Marmosets for Breeding

When breeding marmosets, researchers consider factors like age and health. They also try to keep the group genetically diverse. To create new breeding pairs, they introduce the monkeys carefully over several weeks. Unfortunately, the time it takes for these pairings to lead to pregnancy can be quite long-sometimes even up to 530 days. If a pair fights, they may need to be separated and introduced to new partners, which can be stressful for the animals.

Researchers know that certain behaviors in marmosets can help with successful pairings. Male marmosets may offer food and support to females, which can help with fertility. Yet, even if females are with dominant males, they may not always get pregnant. In some cases, couples live together for long periods without reproducing.

Introducing a New Method for Breeding

To help improve breeding Success, researchers designed a new method that allows female marmosets to choose their mates. They created a special setup called the “love maze,” where females could interact with multiple males and show their Preferences. By using this method, researchers found that allowing females to pick their partners significantly increased the chances of successful Mating and reduced the time it took to conceive.

Experiment Setup

In the study, twelve young female marmosets participated, and they had the chance to meet twenty-three male marmosets. The love maze was set up to give the females a safe space to explore and show preferences for potential mates. The females could move freely while the males were kept in separate areas nearby. The test allowed each female to display behaviors that indicated which male she preferred.

The maze was designed to ensure a safe exploration environment, minimizing risks of aggression. The females were allowed to spend time around different males to see which one they felt most comfortable with. Researchers noted the females’ behaviors, such as spending time close to a male, which indicated their choice.

Observing Preferences

The females displayed a variety of mating-related behaviors during the tests. They showed interest through actions like tongue flicking, grooming, and sitting close to one male compared to others. This allowed researchers to see which male the female preferred. The researchers also recorded how much time the females spent with each male.

Through the love maze, researchers allowed the females to express their interests openly, and the females quickly indicated their preferred partners. They used the time spent near each male as a key indicator to determine their choice.

Results of the Study

The study found that giving females a choice led to a higher rate of successful pregnancies. Out of the females who confirmed a preference for a male, many were pregnant within 90 days. On the other hand, those that were just randomly paired with males showed much lower rates of pregnancy success.

Overall, when females were able to choose their mates, it significantly increased the likelihood of conception. This showed how important it is for females to have the freedom to select their partners in a breeding context.

Marmoset Social Behaviors

Marmosets are known for forming strong, lasting bonds when they mate. In the wild, they can be flexible in their mate choices and often engage in mating outside their primary partner. In captivity, this study showed that females can still choose which male they want, even if they may face challenges from dominant males.

Female marmosets in the love maze expressed their preferences clearly. While males displayed competing behaviors to attract the females, the females decisively chose their mates, leading to significant differences in reproductive success.

Conclusion

The findings from the study on marmosets have important implications for research. Allowing females to select their breeding partners leads to better outcomes for pregnancy and offspring survival. The love maze offers a practical and effective method for supporting breeding in research facilities.

These insights can help foster sustainable marmoset populations in laboratories. The study highlights that female choice plays an essential role in breeding success and suggests that future research should consider these dynamics. By giving females the opportunity to select their partners, researchers can establish healthier, more productive breeding colonies that contribute to advancements in biomedical research.

Original Source

Title: Improving reproductive success in captive marmosets through active female choice

Abstract: The recent upsurge in the use of common marmosets (Callithrix jacchus) as a desirable model for high priority biomedical research has challenged local and global suppliers struggling to provide sufficient numbers of marmosets for large scale projects. Scientific research laboratories are increasingly establishing institutional breeding colonies, in part to combat the resulting shortage and high cost of commercially available animals, and in part to have maximum control over research lines involving reproduction and development. For such laboratories, efficient marmoset breeding can be challenging and time consuming. Random male/female pairings are often unsuccessful, with intervals of several months before attempting alternate pairings. Here we address this challenge through a behavioral task that promotes self-directed female selection of potential mates to increase the efficiency of breeding in captive marmosets. We created a partner preference test ( love maze) in which nulliparous females (n=12) had the opportunity to select between two eligible males (n=23) at a time, in a forced choice test. In this test, both males usually displayed sexual solicitations. However, the female would clearly indicate her preference for one. Most commonly, the female actively ignored the non-preferred male and directed overt prosocial behaviors (e.g. proceptive tongue-flicking, approach and grooming) to the preferred male. Moreover, once a male was selected in this context, the female would continue to prefer him over other males in three consecutive testing sessions. Compared with random pairings, this directed female choice showed a 2.5-fold improvement in breeding within 90 days compared to random pairings. This cost-effective and straightforward pairing practice can be used to enhance breeding efficiency in both small and large marmoset colonies.

Authors: Yogita Chudasama, T. M. Drazan, S. P. Bradley, A. Jones, K. Allen-Worthington

Last Update: 2024-05-09 00:00:00

Language: English

Source URL: https://www.biorxiv.org/content/10.1101/2024.05.08.593247

Source PDF: https://www.biorxiv.org/content/10.1101/2024.05.08.593247.full.pdf

Licence: https://creativecommons.org/publicdomain/zero/1.0/

Changes: This summary was created with assistance from AI and may have inaccuracies. For accurate information, please refer to the original source documents linked here.

Thank you to biorxiv for use of its open access interoperability.

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