Studying Sand Dollar Larvae with Microfluidics
Research reveals how sand dollar larvae use cilia for feeding and movement.
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Table of Contents
The study of small marine animals, like sand dollar larvae, helps us learn more about their development and behavior. Sand dollars are sea creatures that go through several stages in life, starting as small larvae that float around in the ocean before they become adult, bottom-dwelling animals. During their time as larvae, they need to feed and move around, which they do using tiny hair-like structures called cilia.
This research focuses on how these larvae move in water and how they use their cilia to feed. By understanding how sand dollar larvae interact with the water around them, we can gather important information that impacts both biology and engineering.
Feeding and Movement
The Role of Cilia inCilia are small, hair-like structures that cover the surface of sand dollar larvae. These structures beat in a coordinated way to create water movement. This movement helps the larvae feed by allowing them to capture small algae, which provide the nutrients they need to grow. Cilia also help the larvae swim by pushing water behind them, allowing them to move through their environment.
Feeding is particularly crucial during the planktonic stage since sand dollar larvae rely on tiny algae that may not always be available in large amounts. When food is scarce, larvae may struggle to grow, which can lead to longer periods of being a Larva and higher chances of dying. Thus, researching how they use cilia for feeding and movement is important in understanding their survival and overall population health.
Challenges in Studying Larval Behavior
Studying these tiny creatures in their natural environment can be difficult. Researchers often need to rely on laboratory methods to better understand how sand dollar larvae behave when feeding and moving. By studying them in controlled conditions, scientists can isolate variables and focus on specific aspects of their behavior.
One useful method in this research is called Microfluidics. This technique involves using tiny channels to control the flow of water, allowing scientists to mimic ocean conditions. By combining microfluidics with advanced imaging techniques, researchers can observe how cilia work and how larvae respond to different water flows and food conditions.
Overview of Microfluidics
Microfluidics is the study of how small amounts of fluids move through tiny channels. In this research, microfluidics allows scientists to create a controlled environment where they can study sand dollar larvae. This setup lets researchers introduce various conditions, like different food levels and water flows, while observing the larvae’s reactions.
Using microfluidic devices, researchers can closely analyze the Hydrodynamics, or the movement of water, around sand dollar larvae. This analysis can help uncover the relationship between the physical environment and the behavior of these young sea creatures.
Designing the Study
To study sand dollar larvae, researchers designed microfluidic devices that are long, narrow channels where the larvae could live and be observed. These devices allow scientists to introduce small particles that can mimic food and visualize how the larvae move and eat.
In this study, the sand dollar larvae were raised in a marine lab. Researchers collected adult sand dollars from the ocean and induced them to release eggs and sperm to create fertilized larvae. Once the larvae developed, they were placed into the microfluidic channels for observation.
Observing Sand Dollar Larvae Behavior
Researchers examined the larvae at various stages of their development. At different points, they observed how well the larvae could feed and how their movements changed as they aged. By using colored beads in the water, scientists could visualize the flow patterns created by the cilia.
The study involved tracking the velocities (speed and direction) of the beads in the water to infer how effectively the larvae were creating water movement around them. This process helped the researchers understand how ciliary movements contributed to feeding and overall hydrodynamics in the water.
Key Findings from the Research
One of the significant findings was that sand dollar larvae that were fed more had different ciliary patterns and overall behaviors compared to those that were low-fed. The well-fed larvae showed faster growth and shorter larval duration. In contrast, low-fed larvae had longer arms, which may help them capture more food but also resulted in a slower development.
The researchers also found that the hydrodynamic patterns produced by the cilia were more pronounced in well-fed larvae. This means that these larvae were better at creating the water movement needed to feed effectively, showcasing a direct relationship between food availability and the efficiency of feeding mechanisms.
Importance of Study
Understanding how sand dollar larvae use cilia to feed and swim is essential for several reasons. It helps shed light on the survival strategies of these organisms during their early life stages. Insights gained from this research can also guide broader studies regarding marine biology and environmental changes that affect marine life.
Furthermore, these observations can inspire designs in engineering. Biomimicry, which involves emulating nature's strategies to solve human challenges, could benefit from insights gained from the hydrodynamic behaviors of sand dollar larvae. Learning how these organisms effectively feed in low-food environments could lead to the creation of innovative technologies in fluidic systems and robotics.
Future Directions
This research opens several avenues for future work. There is potential for further exploration of how different environmental conditions, such as temperature and salinity, affect the behavior of sand dollar larvae. Additionally, researchers could investigate how changes in the availability of food or pollutants in the water might impact their growth and survival.
By continuing to refine microfluidic techniques, scientists can create even more elaborate experiments that will deepen their understanding of marine larvae. This way, they can develop better conservation strategies for marine ecosystems, particularly in light of climate change and human impacts on the environment.
Conclusion
The study of sand dollar larvae using microfluidics allows for a detailed investigation of their feeding and movement behaviors. By examining how these small creatures interact with their water environment, researchers can learn more about marine biology, the strategies larval organisms use to thrive, and how engineering can take inspiration from nature.
This research emphasizes the importance of larval stages in the life cycles of marine organisms and highlights the need for continued exploration of how these stages influence adult populations. Understanding these early life stages will ultimately enhance our knowledge of marine ecosystems and inform conservation efforts for future generations.
Title: Microfluidics for Hydrodynamics Investigations of Sand Dollar Larvae
Abstract: The life cycle of most marine invertebrates includes a planktonic larval stage before metamorphosis to bottom-dwelling adulthood. During larval stage, ciliary-mediated activity enables feeding (capture unicellular algae) and transport of materials (oxygen) required for the larva's growth, development, and successful metamorphosis. Investigating the underlying hydrodynamics of these behaviors is valuable for addressing fundamental biological questions (e.g., phenotypic plasticity) and advancing engineering applications. In this work, we combined microfluidics and fluorescence microscopy as a miniaturized PIV (mPIV) to study ciliary-medicated hydrodynamics during suspension feeding in sand dollar larvae (Dendraster excentricus). First, we confirmed the approach's feasibility by examining the underlying hydrodynamics (vortex patterns) for low- and high-fed larvae. Next, ciliary hydrodynamics were tracked from 11 days post-fertilization (DPF) to 20 DPF for 21 low-fed larvae. Microfluidics enabled the examination of baseline activities (without external flow) and behaviors in the presence of environmental cues (external flow). A library of qualitative vortex patterns and quantitative hydrodynamics was generated and shared as a stand alone repository. Results from mPIV (velocities) were used to examine the role of ciliary activity in transporting materials (oxygen). Given the laminar flow and the viscosity-dominated environments surrounding the larvae, overcoming the diffusive boundary layer is critical for the organism's survival. Peclet number analysis for oxygen transport suggested that ciliary velocities help overcome the diffusion dominated transport (max Pe numbers between 30-60). Microfluidics serving as mPIV provided a scalable and accessible approach for investigating the ciliary hydrodynamics of marine organisms.
Authors: Wesley A. Chen, Bryant A. Lopez, Haley B. Obenshain, Moses Villeda, Brian T. Le, Brenda AAB. Ametepe, Ariana Lee, Douglas A. Pace, Siavash Ahrar
Last Update: 2023-12-29 00:00:00
Language: English
Source URL: https://arxiv.org/abs/2401.00056
Source PDF: https://arxiv.org/pdf/2401.00056
Licence: https://creativecommons.org/licenses/by-nc-sa/4.0/
Changes: This summary was created with assistance from AI and may have inaccuracies. For accurate information, please refer to the original source documents linked here.
Thank you to arxiv for use of its open access interoperability.