Combining Nature and Science for Immune Health
A look at how chitosan and Alstonia boonei enhance immunity.
Fonye Nyuyfoni Gildas, Eya’ane Meva Francois, Ninpa Kuissi Chris Rosaire, Bamal Hans-Denis, Fannang Simone Veronique, Beglau Thi Hai Yen, N. A. Fetzer Marcus, Ntoumba Agnes Antoinette, Nguemfo Edwige Laure, Hzounda Fokou Jean Batiste, Djuidje Annie Guilaine, Kuinze Kojap Augustine, Tako Djimefo Alex Kevin, Tchangou Njiemou Armel Florian, Evouna Danielle Ines Madeleine, Dongmo Alain Bertrand, Paboudam Gbambie Awawou, Janiak Christoph
― 5 min read
Table of Contents
- Plants and Healing
- Chitosan: A New Hero
- What’s So Special about Alstonia boonei?
- Why Combine Chitosan and Alstonia boonei?
- How Do We Get the Goodies from Alstonia boonei?
- Making the Magical Chitosan Nanocapsules
- Checking It All Out
- Testing on Rats (No, Not the Kind You See in the City)
- What About Side Effects?
- The Results Are In: A Stronger Immune System!
- The Science of Immunomodulation
- The Evidence Keeps Piling Up
- Why Is This So Exciting?
- Wrapping It Up
- Original Source
Immunity is like our body’s own defense team. It protects us from germs and injuries. There are two main types: innate immunity, which is our first line of defense, and acquired immunity, which kicks in with more specific responses. Without this defense, we would be easy targets for all kinds of nasty things.
Plants and Healing
In many parts of the world, especially Africa, people have been using plants to treat illnesses for ages. These plants can help our immune system, which makes them really interesting. One such plant is Alstonia boonei, known locally for its healing properties. This plant has a lot to offer when it comes to fighting off sickness.
Chitosan: A New Hero
Now, let’s talk about chitosan. This is a fancy word for a natural substance made from the shells of crustaceans, like shrimp. Researchers are starting to create tiny packages, called nanocapsules, using chitosan. These nanocapsules can carry medicine or plant extracts directly to where they are needed in the body, kind of like a delivery service.
What’s So Special about Alstonia boonei?
The exciting thing about Alstonia boonei is that it contains many natural compounds that can help with various health issues. Studies show that it has properties that can reduce inflammation, fight infections, and even help with pain. It seems like this plant is quite the overachiever!
Why Combine Chitosan and Alstonia boonei?
Combining chitosan with Alstonia boonei might lead to even better health benefits. The idea is that by putting the plant’s good stuff into chitosan nanocapsules, we can improve how our immune system works. This means helping our body fight off diseases better than ever before. And let’s face it: who wouldn’t want to boost their immune system without side effects?
How Do We Get the Goodies from Alstonia boonei?
To use the healing powers of Alstonia boonei, the first step is to gather the bark of the tree. The bark is dried and then crushed into a fine powder. After this, we mix this powder with a special blend of solvents to extract all the beneficial compounds. Think of this process as making a really potent herbal tea, but a bit more scientific!
Making the Magical Chitosan Nanocapsules
Now that we have our plant extract, the next task is to create the chitosan nanocapsules. By mixing the chitosan with the plant extract and a few other ingredients, we can make these tiny medicine packs. This mix is then stirred around really fast to create the nanocapsules, which are super small and can easily move around in our bodies.
Checking It All Out
Just like a chef tastes their dish, scientists need to check that their nanocapsules are working properly. They look at how much of the plant extract is inside the capsules and how they behave in tests. This is important to make sure that when we take them, we get all the benefits!
Testing on Rats (No, Not the Kind You See in the City)
To see how effective these nanocapsules are, scientists often test them on animals. In this case, young rats are used to check if the capsules really help improve their immune system. It’s like doing a practice run before giving it to humans! The rats are monitored closely to see how they respond.
What About Side Effects?
Nobody wants a surprise when it comes to side effects. So, scientists also conduct safety tests. When the chitosan nanocapsules were given to rats at high doses, there were no major health issues. This means that they can be safe options for future use.
The Results Are In: A Stronger Immune System!
After testing, it seems that the chitosan nanocapsules can actually make the rats' Immune Systems stronger. They showed improvements in various blood parameters, which means that their defenses were boosted. This is fantastic news! It suggests that humans might benefit too when they use these capsules filled with Alstonia boonei extract.
The Science of Immunomodulation
Now that we know the capsules work, let’s talk about why this is important. Immunomodulation is essentially fine-tuning the immune system. If it's too weak, we can boost it. If it's overactive, we can calm it down. Natural substances like those in Alstonia boonei might just help balance everything out!
The Evidence Keeps Piling Up
More evidence shows that natural ingredients can really help our immune functions. Not just through encouraging the body’s natural defenses, but also by working alongside other treatments. Chitosan nanocapsules, paired with plant extracts, may be the next big thing in the world of medicine.
Why Is This So Exciting?
While traditional medications have their place, using natural products offers a way to support our health with fewer side effects. With results showing the effectiveness of chitosan and Alstonia boonei, we might be on the brink of a new approach to treating and preventing diseases.
Wrapping It Up
In the end, this journey through the world of immunity and natural remedies has shown how traditional knowledge and modern science can come together. By focusing on the healing properties of plants and innovative delivery methods like chitosan nanocapsules, we can open up new possibilities for better health.
So, let’s keep our spirits up and our immune systems strong-with a little help from nature!
Title: Chitosan nanocapsules with Alstonia boonei extract modulate the immune system in Wistar rats
Abstract: The development of biosynthetic methods for nanoparticles using plants presents an exciting opportunity to better utilize our rich and diverse medicinal flora. We are particularly interested in creating nanocapsules using Alstonia boonei, a Cameroonian plant known for its immunomodulatory properties. Chitosan nanocapsules were synthesized from the methanol/dichloromethane extract of the powdered stem bark of A. boonei after harvest and drying. The encapsulation of the secondary metabolites was achieved using the ionic gelation method, which involved the agitation of a chitosan solution, extract, and tripolyphosphate. Subsequently, the encapsulation efficiency was calculated. Infrared spectroscopy identified the various functional groups present in the nanocapsules. The acute toxicological profile of these chitosan nanocapsules at a limit dose of 2000 mg/kg, along with their immunomodulatory activities, was evaluated in Wistar rats. The immunomodulatory potential was assessed in dexamethasone-induced immunosuppressed rats by measuring total blood count, delayed-type hypersensitivity response, and hemagglutinating antibody titre between groups of animals after 14 days of treatment. The data collected on the synthesis and characterization confirmed the formation of nanocapsules. This was evidenced by infrared spectroscopy and an entrapment efficiency of 69%. Powder X-ray diffraction confirmed the presence of chitosan in the polymer material. SEM imaging further confirmed the formation of nanocapsules. The toxicological profile of these nanocapsules was found to be satisfactory. Administration of chitosan nanocapsules containing Al. boonei methanol/dichloromethane extracts at doses of 100 mg/kg, 200 mg/kg, and 500 mg/kg body weight significantly prevented dexamethasone-induced immunosuppression in rats. This was achieved by increasing the parameters of total blood count (hematocrit, mean corpuscular volume, platelets, lymphocytes, and granulocyte counts), hemagglutinating antibody titre values, and delayed type hypersensitivity response induced by chicken red blood cells. However, doses of 500 mg/kg of crude A. boonei extract and 500 mg/kg body weight of empty chitosan nanocapsules did not show this effect. The nanocapsules generated from the extracts of chitosan and A. boonei are responsible for immunostimulatory activity and possess therapeutic potentials for the prevention of depressed immune depressed conditions with satisfactory safety at acute dose.
Authors: Fonye Nyuyfoni Gildas, Eya’ane Meva Francois, Ninpa Kuissi Chris Rosaire, Bamal Hans-Denis, Fannang Simone Veronique, Beglau Thi Hai Yen, N. A. Fetzer Marcus, Ntoumba Agnes Antoinette, Nguemfo Edwige Laure, Hzounda Fokou Jean Batiste, Djuidje Annie Guilaine, Kuinze Kojap Augustine, Tako Djimefo Alex Kevin, Tchangou Njiemou Armel Florian, Evouna Danielle Ines Madeleine, Dongmo Alain Bertrand, Paboudam Gbambie Awawou, Janiak Christoph
Last Update: 2024-11-01 00:00:00
Language: English
Source URL: https://www.biorxiv.org/content/10.1101/2024.10.30.621048
Source PDF: https://www.biorxiv.org/content/10.1101/2024.10.30.621048.full.pdf
Licence: https://creativecommons.org/licenses/by-nc/4.0/
Changes: This summary was created with assistance from AI and may have inaccuracies. For accurate information, please refer to the original source documents linked here.
Thank you to biorxiv for use of its open access interoperability.