Simple Science

Cutting edge science explained simply

# Biology # Microbiology

The Hidden Role of Yeasts on Seeds

Exploring the significance of yeasts associated with seeds and their impact on plant health.

Muriel Marchi, Anaïs Bosc-Bierne, Thomas Lerenard, Josiane Le Corff, Sophie Aligon, Aurélia Rolland, Marie Simonin, Coralie Marais, Martial Briand, Viviane Cordovez, Linda Gouka, Thomas Guillemette, Philippe Simoneau, Natalia Guschinskaya

― 6 min read


Yeasts: Key Players in Yeasts: Key Players in Seed Health crucial agricultural roles. Examining yeasts on seeds and their
Table of Contents

Plants don't live in isolation. Just like us, they have friends and even a few foes that help shape their lives. These "friends" are tiny Microorganisms, especially the ones found around roots and leaves. These little helpers can make a plant healthier, help it get more nutrients, handle stress better, and even defend against diseases. Recently, scientists have started looking into the less popular crowd-the microorganisms that hang out on Seeds.

What's So Special About Seeds?

Seeds are the starting point for most plants. They’re like the starter kit for growing crops and ensuring food production. Despite their importance, not many scientists have looked closely at what goes on with the microorganisms associated with seeds. There's plenty of previous research focused on root and leaf microorganisms, but seed friends are still under the radar. Only a few studies have explored how these tiny pals influence seed germination, growth, and resistance to specific diseases.

Yo, Where Are the Fungi?

Most of the research around seed microorganisms has centered on bacteria, leaving fungi out of the conversation. Yet, seeds are like a mini-microbial party, hosting both fungi and bacteria in roughly equal numbers. A recent study revealed that there are over 2000 types of fungi living on seeds across various plant species. Some of these fungi are quite common, appearing in multiple types of plants. Interestingly, a handful of these fungi are actually YEASTS.

Yeast: The Unsung Heroes

You might know yeast as the stuff that makes bread rise and helps produce beer. But these little fungi can also be beneficial for plants. Despite their potential, not much has been done to study yeast in agriculture. Most research has focused on yeast’s role in food and medicine. It’s high time we give these tiny organisms the spotlight they deserve in farming.

Investigating Seed-Associated Yeast

This study aimed to dive into the diversity of yeasts found on seeds of various plants. Scientists set out to answer some key questions: What types of yeast are hanging out on seeds? Are there particular yeast types unique to certain plants? And how well do our findings match up with what has been found in previous research?

To investigate these questions, scientists gathered seeds from a mix of wild plants and crops. They used various methods, including traditional culturing techniques and modern genetic analysis, to identify the yeasts present.

Getting Down to Business: Yeast Isolation

The team isolated yeasts from seeds using two primary methods. The first was a classic technique where they placed seeds on special agar plates and waited for the yeasts to grow. The second method involved soaking seeds and seedlings in a solution to help collect the microorganisms more efficiently. This combination of methods allowed researchers to capture a wider array of yeasts.

Sorting Out the Yeasts

To identify the yeasts, scientists analyzed their DNA. This involved using specific markers that let them pinpoint which yeast types were present. They compared their findings against a larger database of seed yeast information to see how their samples matched up with previously identified strains.

Diverse Yeast Collection

After all the hard work, the scientists managed to isolate a whopping 219 different yeast strains. They identified these yeasts as part of two main groups: Ascomycota and Basidiomycota. Notably, most of the yeasts came from the Basidiomycota group, which was quite common.

Among the strains found, a few types stood out because they were particularly abundant. Some genera like Holtermaniella, Vishniacozyma, Naganishia, and Filobasidium had dozens of isolates. However, some yeasts were rare and only appeared once or twice in the samples.

A Closer Look at Yeast Morphology

The scientists didn’t just want to know which yeasts were there; they also wanted to look at what they actually looked like. They examined the yeast colonies under the microscope, noting their texture, size, and color. Most yeast colonies were smooth, with some showing off vibrant colors, such as reds and yellows.

Yeast Habitat Specificity

Interestingly, most of the yeasts came from seeds rather than seedlings. Some yeast types were found only on specific plants-such as Naganishia and Rhodotorula, which were exclusive to one type of crop. This kind of specificity suggests that some yeasts have become well-adapted to their plant hosts.

The Treasure Trove of Seed Microbiota

By comparing their findings to a broader database, researchers discovered that many of their yeast isolates matched previously identified core taxa. Some of these yeasts were common across many plant species, indicating that these microorganisms play significant roles in seed health.

Yeasts: The Agricultural Game-Changers

The findings from this study highlight a treasure trove of opportunities in the agricultural world. Yeasts can be seen as the underappreciated allies of agriculture. With the right focus and research, we can learn how to use these microorganisms to help crops grow better, resist diseases, and become more resilient in the face of environmental challenges.

The Path Forward

The study's findings pave the way for future research on the roles of these yeasts in Plant Health and growth. Understanding how yeasts interact with plants could lead to new ways of improving crop yields and protecting plants from pests and diseases. Think of it as giving plants their own tiny bodyguards.

Ultimately, the potential for using yeasts in agriculture is vast. With more attention and research, we could explore how to enhance plant health through seed inoculation with beneficial yeast strains. It’s time to open our eyes to these tiny friends and see the huge impact they can have on the world of farming.

In Summary

The world of microorganisms around plants is rich and diverse. While bacteria have taken the spotlight for some time, yeasts deserve their moment too. This study sheds light on the unique and varied yeasts found on seeds, highlighting their possible roles in plant health. As we explore more about these little fungi, we might just find new ways to benefit crops and, ultimately, our food supply. Who knew that such tiny organisms could be so mighty?

So, the next time you’re baking bread or enjoying a brew, remember there’s a whole world of yeast out there, making waves in agriculture, one seed at a time!

Original Source

Title: Unexplored Yeast diversity in Seed Microbiota

Abstract: Yeasts are known to be fantastic biotechnological resources for medical, food, and industrial applications, but their potential remains untapped in agriculture, especially for plant biostimulation and biocontrol. In particular, yeasts have been reported as part of the core microbiome of seeds using next generation sequencing methods, but their diversity and functional roles remain largely undescribed. This study aimed to fill this knowledge gap by characterizing the diversity of seed-associated yeasts across nine plant species (crops and non-cultivated species) using culturomics, microscopy, and metabarcoding. Our results show that seed-associated yeasts largely belong to Basidiomycota phylum and more particularly to the Tremellomycetes class. This yeast collection covers 15 genera (2 of Ascomycota and 13 of Basidiomycota). Out of the 219 isolates described, the most frequently isolated yeasts were Holtermaniella, Vishniacozyma, Filobasidium, Naganishia and Sporobolomyces. The yeasts from these dominant genera were isolated from multiple plant species (4 to 8), except for Naganishia which only originated from Solanum lycopersicum L.. These results are also consistent with the fact that these dominant taxa were recently identified as members of the core seed microbiome, indicating their high prevalence and abundance across diverse plant hosts and environments. Compared to previous plant yeast diversity surveys, the members from Ascomycota are less frequent in seeds and only represented here by the Aureobasidium and Taphrina genera. Altogether, these results suggest that yeasts are generally well-adapted to the aboveground habitats of plants, but seeds represent a specific habitat that diverse Basidiomycota yeasts can colonize. Take away messageO_LI219 yeasts isolated from seeds and seedlings of diverse plant species C_LIO_LIMost isolates are Basidiomycota yeasts, especially of the Tremellomycetes class C_LIO_LIAscomycota yeasts are rarely isolated from the seed environment C_LIO_LIThe most frequently isolated yeasts belong to Holtermaniella, Vishniacozyma, Filobasidium and Sporobolomyces genera C_LIO_LIThe collection is representative of taxa found in seed microbiota of multiple plant species C_LI

Authors: Muriel Marchi, Anaïs Bosc-Bierne, Thomas Lerenard, Josiane Le Corff, Sophie Aligon, Aurélia Rolland, Marie Simonin, Coralie Marais, Martial Briand, Viviane Cordovez, Linda Gouka, Thomas Guillemette, Philippe Simoneau, Natalia Guschinskaya

Last Update: Nov 27, 2024

Language: English

Source URL: https://www.biorxiv.org/content/10.1101/2024.11.27.625647

Source PDF: https://www.biorxiv.org/content/10.1101/2024.11.27.625647.full.pdf

Licence: https://creativecommons.org/licenses/by/4.0/

Changes: This summary was created with assistance from AI and may have inaccuracies. For accurate information, please refer to the original source documents linked here.

Thank you to biorxiv for use of its open access interoperability.

Similar Articles