Cinnamon and Chitosan: A New Hope for Rice Farmers
Natural solutions like cinnamon may help fight rice diseases.
Qamar Mohammed Naji, Dzarifah Mohamed Zulperi, Khairulmazmi Ahmad, Erneeza Mohd Hata
― 6 min read
Table of Contents
Rice is one of the most important foods for humans. People have been growing rice for thousands of years, with evidence of its cultivation going back to around 1500-1000 BC. Today, about 40% of the world's population relies on rice as a staple food, especially in countries that are still developing. Rice grows best in warm and humid conditions, which are found in many Asian countries, especially tropical ones.
In Malaysia, rice is the third most crucial crop after rubber and palm oil, with the states of Kedah and Perlis being the main places where rice is grown. However, rice fields face many problems, mostly from diseases caused by germs, fungi, and bacteria. These diseases can seriously reduce how much rice farmers can produce. There are over 70 diseases known to affect rice, with several major bacterial diseases that attack various parts of the rice plant, including the seeds, leaves, and roots.
One of the most severe bacterial diseases impacting rice globally, including in Malaysia, is bacterial panicle blight (BPB). This disease is caused by a bacterium named Burkholderia glumae. If rice plants get this disease, it can result in a terrible yield loss, sometimes up to 75%. B. glumae loves warm weather and can grow in temperatures up to 41°C. It infects rice seeds, causing problems as the seedlings grow. The bacteria produce harmful substances that can lead to rot in the seedlings.
Farmers and scientists are trying different methods to manage B. glumae in rice fields. Some of these methods include using harmless strains of Burkholderia to reduce toxins in the soil or using seeds that are free from pathogens. Chemical treatments have also been used to fight against diseases, but they can have negative effects on the soil and plants, making them less popular. Recently, natural remedies like cinnamon bark have shown promise as Antibacterial solutions to combat B. glumae.
The Wonder of Cinnamon
Cinnamon is a spice that many people love to use in their cooking. It comes from the inner bark of certain trees and has been valued not just for its taste but also for its medicinal properties. Cinnamon bark extract is loaded with active compounds that have various health benefits, such as fighting fungi and bacteria, reducing inflammation, and even having potential cancer-fighting properties.
With ongoing advancements in technology, researchers are exploring the use of natural ingredients, like cinnamon, in fighting plant diseases. One area of research focuses on tiny particles known as organic nanoparticles (ONPs). These small particles can help deliver natural remedies to plants more effectively. One promising natural material for creating these nanoparticles is Chitosan, which comes from the shells of crustaceans or fungi.
Chitosan has many advantages: it's natural, safe for the environment, and can be altered chemically to improve its effectiveness. These nanoparticles can carry both water-soluble and fat-soluble compounds, making them very useful in agriculture.
Creating a New Solution
In a study to tackle the problem of bacterial panicle blight, researchers aimed to develop a new way to use cinnamon bark extract combined with chitosan nanoparticles. The goal was to create a product that could effectively fight off B. glumae in rice plants.
To start, researchers gathered the necessary materials, including chitosan and cinnamon bark. The cinnamon bark was ground into a powder, soaked in a solution, and filtered to produce a concentrated extract. This extract was then combined with chitosan to create nanoparticles designed to deliver the antibacterial properties of cinnamon directly to the rice plants.
Understanding the Ingredients
The research involved detailed analyses to identify the different active components in the cinnamon bark extract. This was done using a method called gas chromatography/mass spectrometry (GC-MS). The results showed that the extract contained several natural compounds, with the most common being (Z)-3-Phenylacrylaldehyde. These compounds contribute to the antibacterial activities of cinnamon, making it a key player in the fight against plant diseases.
The next step in the research was to characterize the newly created nanoparticles. This meant measuring their size, stability, and how well they could carry the cinnamon bark extract. Researchers found that the size of the nanoparticles could be changed by altering the concentration of a specific ingredient used in the process. This showed that careful adjustments could lead to better stability and effectiveness.
The Antibacterial Test
Once the nanoparticles were created, the researchers needed to see how well they worked against B. glumae. They conducted experiments in the lab to test the antibacterial properties of the CBE-CS formulation made from cinnamon bark extract and chitosan nanoparticles.
During these tests, they spread a bacterial suspension on culture plates and placed disks containing the new formulation on the plates. After letting them sit for a while, they measured the area around the disks where bacteria couldn’t grow, known as the "zone of inhibition." The results showed that the formulation could effectively reduce the growth of B. glumae, particularly when a specific amount of the crosslinking agent was added.
Time to Assess Effectiveness
The researchers also wanted to see how quickly the CBE-CS formulation could kill the bacteria. They set up tests to monitor bacterial growth over time using different concentrations of the formulation. The results revealed that the new antifungal solution significantly slowed down the growth of B. glumae, especially at higher concentrations.
This is helpful for farmers because using a natural product that effectively reduces bacterial growth can lead to healthier rice plants and better yields. As an added bonus, it’s a more environmentally friendly approach compared to using some synthetic chemicals.
Conclusion
The research points to a promising new use for cinnamon bark extract combined with chitosan nanoparticles in fighting bacterial diseases in rice cultivation. This innovative approach allows for effective delivery of natural antibacterial properties to the plants. Given the ongoing challenges in agricultural disease management, particularly in rice production, this method could pave the way for healthier crops while being safe for the environment.
So, next time you sprinkle cinnamon on your morning toast, think of it not just as a tasty addition but as a potential superhero against plant diseases! With the right research and applications, even a humble spice could make a big difference in feeding the world.
Title: Nanobactericides Derived from Cinnamon Bark Extract: Phytochemical Profiling and Antibacterial Efficacy Against Bacterial Panicle Blight in Rice
Abstract: Bacterial panicle blight (BPB), caused by the Gram-negative aerobic bacterium Burkholderia glumae (B.glumae), poses a significant threat to global rice production. Cinnamon bark extract (CBE) has demonstrated potent antioxidant and antimicrobial properties due to its high concentration of bioactive compounds, including eugenol and cinnamaldehyde. To enhance the efficacy and stability of these volatile compounds, this study employed nanotechnology and encapsulation techniques. The objective was to develop a CBE-based nanoformulation to inhibit B. glumae and control BPB in rice. CBE-chitosan (CBE-CS) nanoformulations were synthesized using ionic cross-linking between chitosan and trisodium phosphate (TPP) at various concentrations (0%, 0.5%, 1%, 2%, and 4% TPP). More than 15 active compounds were identified in CBE, including (Z)-3-Phenylacrylaldehyde, 2-Propenoic acid, 3-(2-hydroxyphenyl), cinnamaldehyde dimethyl acetal, and hexadecanoic acid. Bacterial membrane damage was significantly greater in treatments with CBE compared to untreated controls. The synthesized nanoparticles ranged in size from 43.66 nm to 106.1 nm, with encapsulation efficiencies between 48.65% and 48.78%, and loading capacities between 25.65% and 33.9%. Scanning electron microscopy (SEM) revealed spherical and homogeneous nanoparticles, while FTIR and XRD analysis confirmed the successful encapsulation of CBE in the chitosan nanoparticles. The antibacterial activity of the nanoformulations showed inhibition zones ranging from 7.5 to 11.8 mm, with the CBE-CS formulation containing 0.5% TPP demonstrating the highest efficacy (MIC = 15.6 mol/ml; MBC = 31.25 mol/ml).
Authors: Qamar Mohammed Naji, Dzarifah Mohamed Zulperi, Khairulmazmi Ahmad, Erneeza Mohd Hata
Last Update: 2024-12-08 00:00:00
Language: English
Source URL: https://www.biorxiv.org/content/10.1101/2024.12.04.626863
Source PDF: https://www.biorxiv.org/content/10.1101/2024.12.04.626863.full.pdf
Licence: https://creativecommons.org/licenses/by/4.0/
Changes: This summary was created with assistance from AI and may have inaccuracies. For accurate information, please refer to the original source documents linked here.
Thank you to biorxiv for use of its open access interoperability.